Overview

  • Product nameAnti-GPCR GPR15 antibody
    See all GPCR GPR15 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    YYATSPNSDIRETHSH

    , corresponding to N terminal amino acids 13-28 of Human GPCR GPR15.

  • Positive control
    • Jurkat, A549 or K562 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab16445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 40 kDa.Can be blocked with GPCR GPR15 peptide (ab6101). Detects a band of approximately 50 kDa in human heart tissue lysate.

Target

References for Anti-GPCR GPR15 antibody (ab16445)

ab16445 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3 cell line)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 5 µg
Treatment Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification NIH-3T3 cell line
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Oct 15 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cytospined HEK293 human cells)
Permeabilization No
Specification Cytospined HEK293 human cells
Fixative Any
Username

Dr. Francois Daubeuf

Verified customer

Submitted Oct 13 2015

Application Western blot
Sample Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 5 µg
Treatment Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification THP-1, A549, HEK293, CEMx-174
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Oct 09 2015

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cell from bone marrow)
Specification Cell from bone marrow
Gating Strategy Alive cells (DAPI-), blood cells excluded
Preparation Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

Submitted Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"