Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR15
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 13 to 28 of human GPR15 (1). The sequence differs from those ofAfrican green monkey and pig-tailed macaque BOB byone amino acid (2)

  • General notes
    Stable for one year.


    Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein coupled receptors including CCR5, CXCR4, CCR3,CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively) were recently cloned. This novel G protein-coupled receptor serves as coreceptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein coupled receptors including CCR5, CXCR4, CCR3,CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively) were recently cloned. This novel G protein-coupled receptor serves as coreceptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 50 kDa.Can be blocked with GPCR GPR15 peptide (ab6101).

Target

Anti-GPCR GPR15 antibody images

  • All lanes : Anti-GPCR GPR15 antibody (ab8104)

    Lane 1 : Human heart lysate in absence of specific peptide
    Lane 2 : Human heart lysate in presence of specific peptide
    Lane 3 : Human heart lysate in presence of unrelated peptide


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Lane 1 : Anti-GPCR GPR15 antibody (ab8104) at 0.5 µg/ml
    Lane 2 : Anti-GPCR GPR15 antibody (ab8104) at 1 µg/ml

    Lane 1 : Human spleen lysate
    Lane 2 : Human spleen lysate


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-GPCR GPR15 antibody (ab8104)

This product has been referenced in:
  • Pan B  et al. G-protein coupled receptor 15 mediates angiogenesis and cytoprotective function of thrombomodulin. Sci Rep 7:692 (2017). WB . Read more (PubMed: 28386128) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Caroline Schmutz

Verified customer

Submitted Dec 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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