Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat GPCR GPR17.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Kidney; Mouse Kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 38 kDa).

Target

  • Function
    Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia.
  • Tissue specificity
    Expressed in brain, kidney, heart and umbilical vein endothelial cells. Highest level in brain.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M18273 antibody
    • G protein coupled receptor 17 antibody
    • G-protein coupled receptor 17 antibody
    • GPR 17 antibody
    • Gpr17 antibody
    • GPR17_HUMAN antibody
    • P2Y like receptor antibody
    • P2Y-like receptor antibody
    • Probable P2Y purinoceptor GPR 17 antibody
    • Probable P2Y purinoceptor GPR17 antibody
    • R12 antibody
    • UDP/CysLT receptor antibody
    • Uracil nucleotide/cysteinyl leukotriene receptor antibody
    see all

Images

  • All lanes : Anti-GPCR GPR17 antibody (ab111472) at 1 µg/ml

    Lane 1 : Kidney (Rat) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 14 kDa, 31 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    GPCR GPR17 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of GPCR GPR17 is 38 kDa (SwissProt), however we expect to observe a banding pattern around 41 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab111472 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab111472.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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