Overview

  • Product nameAnti-GPCR GPR20 antibody
    See all GPCR GPR20 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR20
  • Specificityab75559 detects endogenous levels of total GPCR GPR20 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal sequence of human GPCR GPR20

  • Positive control
    • IHC-P: human brain tissue WB: extracts from LOVO cells ICC/IF: A549 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionOrphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
  • Tissue specificityUbiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 20 antibody
    • G-protein coupled receptor 20 antibody
    • Gpr20 antibody
    • GPR20_HUMAN antibody

Anti-GPCR GPR20 antibody images

  • All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
  • Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-GPCR GPR20 antibody (ab75559)

ab75559 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75559.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"