Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR20
  • Specificity
    ab75559 detects endogenous levels of total GPCR GPR20 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal sequence of human GPCR GPR20

  • Positive control
    • IHC-P: human brain tissue WB: extracts from LOVO cells ICC/IF: A549 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
  • Tissue specificity
    Ubiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 20 antibody
    • G-protein coupled receptor 20 antibody
    • Gpr20 antibody
    • GPR20_HUMAN antibody

Images

  • All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
  • Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.

References

ab75559 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75559.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up