The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Proteinase K antigen retrieval is recommended.
FunctionOrphan receptor. Possible candidate for amine-like G-protein coupled receptor.
Tissue specificityHighly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab117106 at 10µg/ml staining GPCR GPR27 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab117106 at 10µg/ml staining GPCR GPR27 in Formalin-fixed, Paraffin-embedded Human Uterus tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-GPCR GPR27 antibody (ab117106)
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