The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IHC-P with human Formalin/PFA-fixed paraffin-embedded tissues requires proteinase K digestion
FunctionOrphan receptor. Possible candidate for amine-like G-protein coupled receptor.
Tissue specificityHighly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.