Anti-GPCR GPR3 antibody (ab106589)

Overview

  • Product nameAnti-GPCR GPR3 antibody
    See all GPCR GPR3 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR3
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 13 amino acids from near the C terminus of Human GPR3 (P46089).

  • Positive control
    • EL4 cell lysate, Human spleen tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionOrphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress (By similarity), amyloid-beta peptide generation in neurons and neurite outgrowth (By similarity). Maintains also meiotic arrest in oocytes.
  • Tissue specificityExpressed predominantly in the central nervous system, and at low levels in the lung, kidney, testis, ovary and eye. Highly expressed in regions of the brain implicated in the Alzheimer disease.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACCA antibody
    • ACCA orphan receptor antibody
    • Adenylate cyclase constitutive activator antibody
    • G protein coupled receptor 3 antibody
    • G-protein coupled receptor 3 antibody
    • Gpr3 antibody
    • GPR3_HUMAN antibody
    • Probable G protein coupled receptor GPR3 antibody
    see all

Anti-GPCR GPR3 antibody images

  • Lane 1 : Anti-GPCR GPR3 antibody (ab106589) at 1 µg/ml
    Lane 2 : Anti-GPCR GPR3 antibody (ab106589) at 2 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 35 kDa
  • ab106589, at 2.5 ug/ml, staining GPCR GPR3 in Human spleen tissue by immunohistochemistry.
  • Immunofluorescence of GPR3 in Human Spleen cells using ab106589 at 20 ug/ml.

References for Anti-GPCR GPR3 antibody (ab106589)

ab106589 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample Mouse Tissue lysate - whole (Mouse whole brain tissue lysate, striatum/cortex)
Specification Mouse whole brain tissue lysate, striatum/cortex
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 05 2013

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo ab81530.
Lamentablemente el anticuerpo no está actualmente en stock, por lo que puede tardar en llegaros entre 1 y 2 semanas.
Si necesitáis cualquier información referente ...

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Gracias por tu respuesta.
Siento mucho que los resultados no hayan mejorado. A pesar de que la garantía ha expirado, al no haber pasado mucho tiempo de ello puedo hacer una excepción y extenderla para enviaros un anticuerpo diferente o un reembols...

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Gracias por mantenerte a la espera, te pido disculpas por ello.
Me comentan mis compañeros del laboratorio que ellos también han observado una banda a elevado peso molecular en homogenados cerebrales. Dado que GPR3 es una proteína membranal “multi...

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Gracias por contactarnos.
Siento mucho que volváis a tener problemas con este anticuerpo, y te agradezco que nos hayas enviado información e imágenes de los resultados. Para tratar de entender mejor el problema te agradecería que me aclararas algu...

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Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Por ello, puedo sugeriros algunas alternativas al protocolo seguido que en ocasiones mejoran los resultados del anticuerpo. De cualquier forma, a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"