Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR31
  • Specificity
    ab75579 detects endogenous levels of total GPCR GPR31 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the internal sequence of human GPCR GPR31.

  • Positive control
    • Extracts from COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab75579 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/5000.

Target

  • Relevance
    GPCR GPR31 is a member of the G protein coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPCR GPR31 is located on human chromosome 6q27.
  • Cellular localization
    Cell membrane; Multi pass membrane protein
  • Database links
  • Alternative names
    • 12-(S)-HETE acid receptor antibody
    • 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor antibody
    • 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor antibody
    • 12-(S)-HYDROXYEICOSATETRAENOIC ACID RECEPTOR antibody
    • 12-HETER antibody
    • bA517H2.2 (G protein coupled receptor 31) antibody
    • G protein coupled receptor 31 antibody
    • GPR31 antibody
    • HETER antibody
    • HETER1 antibody
    • OTTHUMP00000017616 antibody
    • Probable G protein coupled receptor 31 antibody
    see all

Images

  • All lanes : Anti-GPCR GPR31 antibody (ab75579) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from COLO cells with Immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References

This product has been referenced in:
  • Siangjong L  et al. Endothelial 12(S)-HETE vasorelaxation is mediated by thromboxane receptor inhibition in mouse mesenteric arteries. Am J Physiol Heart Circ Physiol 304:H382-92 (2013). WB ; Mouse . Read more (PubMed: 23203967) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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