Overview

  • Product nameAnti-GPCR GPR32 antibody
    See all GPCR GPR32 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR32
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GPCR GPR32 (extracellular).

  • Positive control
    • A549 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/250. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

Anti-GPCR GPR32 antibody images

  • All lanes : Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from A549 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Left panel: immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel: same, after pre-incubation with the immunizing peptide.
  • ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
    Gating Strategy: Forward and side scatter.

    See Abreview

References for Anti-GPCR GPR32 antibody (ab79516)

This product has been referenced in:
  • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Sample Human Cell (Human Primary Small Airway epithelial cell)
Specification Human Primary Small Airway epithelial cell
Gating Strategy Forward and side scatter
Permeabilization No
Username

Dr. Jim Hsiao

Verified customer

Submitted May 15 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (Human Primary Lung Fibroblasts)
Specification Human Primary Lung Fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"