Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR35
  • Host species
    Rabbit
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications
    Suitable for: IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Monkey, Gorilla
  • Immunogen

    Synthetic peptide containing 19 amino acids from the C terminus of Human GPCR GPR35 (UniProt ID: Q9HC97).

  • Positive control
    • Human pancreas tissue; HEK293 Human embryonic kidney cells transfected with GPCR GPR35

Properties

Applications

Our Abpromise guarantee covers the use of ab150635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • Relevance
    G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR35 is a member of this family and is an orphan receptor. GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • G protein coupled receptor 35 antibody
    • G protein coupled receptor 35 antibody
    • GPR 35 antibody
    • GPR35 antibody
    • KYNA receptor antibody
    • Kynurenic acid receptor antibody
    • Probable G protein coupled receptor 35 antibody
    • Probable G protein coupled receptor GPR35 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling GPCR GPR35 with ab150635 at 23 µg/ml after heat induced antigen retrieval.
  • Immunocytochemical analysis of GPCR GPR35 transfected HEK293 Human embryonic kidney cells labeling GPCR GPR35 with ab150635.

References

ab150635 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Rat Cell (dorsal root ganglion neurons)
Specification
dorsal root ganglion neurons
Permeabilization
Yes - PBS+triton X-100 0.1%
Fixative
Paraformaldehyde
Username

Mr. Francesco Resta

Verified customer

Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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