Anti-GPCR GPR35 antibody (ab76217)

Overview

  • Product nameAnti-GPCR GPR35 antibody
    See all GPCR GPR35 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR35
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human GPCR GPR35.

  • Positive control
    • WB: extracts from LOVO cells; ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab76217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • RelevanceG protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR35 is a member of this family and is an orphan receptor. GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach.
  • Cellular localizationCell membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • G protein coupled receptor 35 antibody
    • G protein coupled receptor 35 antibody
    • GPR 35 antibody
    • GPR35 antibody
    • KYNA receptor antibody
    • Kynurenic acid receptor antibody
    • Probable G protein coupled receptor 35 antibody
    • Probable G protein coupled receptor GPR35 antibody
    see all

Anti-GPCR GPR35 antibody images

  • All lanes : Anti-GPCR GPR35 antibody (ab76217) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
  • Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab76217. Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-GPCR GPR35 antibody (ab76217)

ab76217 has not yet been referenced specifically in any publications.

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