Anti-GPCR GPR43 antibody (ab124272)

Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR43
  • Tested applications
    Suitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to the C terminal region of Human GPCR GPR43.

  • Positive control
    • Human tonsil tissue; COS7 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate.
  • Tissue specificity
    Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fatty acid receptor 2 antibody
    • FFA2R antibody
    • Ffar2 antibody
    • FFAR2_HUMAN antibody
    • free fatty acid activated receptor 2 antibody
    • Free fatty acid receptor 2 antibody
    • G protein coupled receptor 43 antibody
    • G protein-coupled receptor 43 antibody
    • G-protein coupled receptor 43 antibody
    • GPR 43 antibody
    • GPR43 antibody
    see all

Images

  • ab124272, at 5 µg/ml, staining GPCR GPR43 in formalin fixed, embedded Human tonsil tissue by Immunohistochemistry.
  • ab124272, at a 1/100 dilution, staining GPCR GPR43 in COS7 cells by iImmunofluorescence.
    The image on the right is treated with the synthesized peptide.

References

ab124272 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The homology of this immunogen sequence to mouse is not good, only about 60%. It is possible yet unlikely that ab124272 will cross react with mouse protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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