Anti-GPCR GPR48 antibody [BBX-3A8] (ab166659)

Overview

  • Product name
    Anti-GPCR GPR48 antibody [BBX-3A8]
    See all GPCR GPR48 primary antibodies
  • Description
    Rat monoclonal [BBX-3A8] to GPCR GPR48
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    cDNA encoding Human GPCR GPR48 (Uniprot: Q9BXB1).

  • Positive control
    • GPCR GPR48-transfected BOSC23 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab166659 was purified by protein G affinity chromatography from cell culture supernatants.
  • Clonality
    Monoclonal
  • Clone number
    BBX-3A8
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Competitive ELISA 1/200 - 1/400.

Target

  • Function
    Orphan receptor.
  • Tissue specificity
    Expressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNMD17 antibody
    • G protein coupled receptor 48 antibody
    • G-protein coupled receptor 48 antibody
    • GPR48 antibody
    • Leucine-rich repeat-containing G protein-coupled receptor 4 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 4 antibody
    • LGR4 antibody
    • LGR4_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of BOSC23 cells labeling GPCR GPR48 with ab166659 at 1.2 µg/106 cells. BOSC23 cells were transiently transfected with an expression vector encoding either Human GPCR GPR48 (red curve), Mouse GPCR GPR48 (blue curve) or an irrelevant protein (control transfectant: black curve), followed by a Phycoerythrin-conjugated secondary antibody.

References

ab166659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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