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Our Abpromise guarantee covers the use of ab71225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/50 - 1/100. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical staining of GPCR GPR49 in Human brain tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71225 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
ab71225 has not yet been referenced specifically in any publications.