Recombinant
RabMAb

Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850)

Overview

  • Product name
    Anti-GPCR GPR49 antibody [EPR3065Y]
    See all GPCR GPR49 primary antibodies
  • Description
    Rabbit monoclonal [EPR3065Y] to GPCR GPR49
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GPCR GPR49 aa 800-900 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab221221)

  • Positive control
    • Fetal skeletal muscle lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 100 kDa.Can be blocked with GPCR GPR49 peptide (ab221221).
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
    • Tissue specificity
      Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
      Contains 16 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FEX antibody
      • G protein coupled receptor 49 antibody
      • G protein coupled receptor 67 antibody
      • g protein-coupled receptor fex antibody
      • G-protein coupled receptor 49 antibody
      • G-protein coupled receptor 67 antibody
      • G-protein coupled receptor HG38 antibody
      • GPR 49 antibody
      • GPR 67 antibody
      • GPR49 antibody
      • GPR67 antibody
      • GRP 49 antibody
      • GRP49 antibody
      • HG 38 antibody
      • HG38 antibody
      • Leucine rich repeat containing G protein coupled receptor 5 antibody
      • Leucine-rich repeat-containing G-protein coupled receptor 5 antibody
      • LGR 5 antibody
      • LGR5 antibody
      • LGR5_HUMAN antibody
      • MGC117008 antibody
      • Orphan G protein coupled receptor HG38 antibody
      see all

    Images

    • Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850) at 1/1000 dilution + Fetal
      skeletal muscle lysate at 10 µg

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa

    References

    This product has been referenced in:
    • Jiang Y  et al. MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/ß-catenin signaling pathway, and enhancing Smac expression. Oncol Lett 15:5833-5840 (2018). Read more (PubMed: 29556310) »
    • Harada Y  et al. Leucine-rich repeat-containing G protein-coupled receptor 5 and CD133 expression is associated with tumor progression and resistance to preoperative chemoradiotherapy in low rectal cancer. Oncol Lett 14:7791-7798 (2017). IHC-P ; Human . Read more (PubMed: 29250176) »

    See all 22 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin)
    Permeabilization
    Yes - citrate
    Specification
    skin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - BSA
    Specification
    Colon
    Blocking step
    Hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Username

    Vinicius Kannen Cardoso

    Verified customer

    Submitted Sep 09 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Ahmar Aziz

    Verified customer

    Submitted Dec 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    none
    Permeabilization
    No
    Sample
    Mouse Cell (colon)
    Specification
    colon
    Gating Strategy
    High FSC/SSC log scale
    Preparation
    Cell harvesting/tissue preparation method: isolate colon, collagenase digestion
    Sample buffer: DMEM
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW620 Colon)
    Loading amount
    100 µg
    Specification
    SW620 Colon
    Treatment
    siRNA - knockdown
    Gel Running Conditions
    Reduced Denaturing (7)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
    Permeabilization
    No
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 12 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
    Permeabilization
    No
    Specification
    colon cancer
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 31 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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