- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-GPCR GPR6 antibody
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DescriptionRabbit polyclonal to GPCR GPR6
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (NASAASLNDS QVVVVAAEGA AAAATAAGGP DTGEWGPPAA AALGAGGGAN) of Human GPCR GPR6 (NP_005275).
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Positive control
- Placenta lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab85005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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FunctionOrphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane. Detected in the intracellular compartments. It is currently unclear whether this is a cell surface or intracellular receptor.
- Information by UniProt
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Database links
- Entrez Gene: 506661 Cow
- Entrez Gene: 2830 Human
- Entrez Gene: 140741 Mouse
- Entrez Gene: 83683 Rat
- Omim: 600553 Human
- SwissProt: P46095 Human
- SwissProt: Q6YNI2 Mouse
- SwissProt: P51651 Rat
see all -
Alternative names
- G protein coupled receptor 6 antibody
- G-protein coupled receptor 6 antibody
- GPR 6 antibody
see all
Images
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Western blot - GPCR GPR6 antibody (ab85005)Anti-GPCR GPR6 antibody (ab85005) at 1 µg/ml + 10 µg Placenta lysate
Secondary
HRP conjugated anti-Rabbit IgG diluted 1/50,000.
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR6 antibody (ab85005)ICC/IF image of ab85005 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85005, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GPCR GPR6 antibody(ab85005)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling GPCR with ab85005 at 5.0µg/ml.
Protocols
References
ab85005 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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