Overview

  • Product name
    Anti-GPCR GPR6 antibody
  • Description
    Rabbit polyclonal to GPCR GPR6
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (NASAASLNDS QVVVVAAEGA AAAATAAGGP DTGEWGPPAA AALGAGGGAN) of Human GPCR GPR6 (NP_005275).

  • Positive control
    • Placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Anti-GPCR GPR6 antibody (ab85005) at 1 µg/ml + 10 µg Placenta lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ICC/IF image of ab85005 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85005, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling GPCR with ab85005 at 5.0µg/ml.

References

ab85005 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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