Anti-GPCR GPR75 antibody (ab75581)

Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR75
  • Specificity
    ab75581 detects endogenous levels of total GPCR GPR75 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human GPCR GPR75.

  • Positive control
    • WB: Extracts from HT-29 and K562 cells ICC/IF: LOVO cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab75581 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Orphan receptor.
  • Tissue specificity
    Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 75 antibody
    • GPR chr2 antibody
    • Gpr75 antibody
    • GPR75_HUMAN antibody
    • GPRchr2 antibody
    • OTTHUMP00000159608 antibody
    • Probable G protein coupled receptor 75 antibody
    • Probable G-protein coupled receptor 75 antibody
    • WI 31133 antibody
    • WI31133 antibody
    see all

Images

  • All lanes : Anti-GPCR GPR75 antibody (ab75581) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from K562 cells
    Lane 3 : Extracts from HT-29 cells with Immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • ab75581, at a 1/500 dilution, staining GPCR GPR75 in LOVO cells by Immunofluorescence, in the absence (left image) and presence (right image) of the immunising peptide.

References

ab75581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

I have been able to contact the lab regarding your question about ab75581.This antibody binds to the cytoplasmic domain of target protein.

I hope this information is helpful to you. Please do not h...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up