The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.
FunctionOrphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
Tissue specificityHigh level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab121390, at 1/25 dilution, staining GPCR GPR78 in paraffin-embedded Human pancreas tissue by Immunohistochemistry, showing distinct cytoplasmic and membranous positivity in intercalated ducts.
Western blot - Anti-GPCR GPR78 antibody (ab121390)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
References for Anti-GPCR GPR78 antibody (ab121390)
This product has been referenced in:
Dong DD et al. GPR78 promotes lung cancer cell migration and metastasis by activation of Gaq-Rho GTPase pathway. BMB Rep49:623-628 (2016).
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