Overview

  • Product name
  • Description
    Goat polyclonal to GPCR GPR81
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QQLARQARMKKATR

    , corresponding to internal sequence amino acids 207-220 of Human GPCR GPR81 (NP_115943.1).

  • Positive control
    • Human liver lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
  • Tissue specificity
    Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Form
    G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
  • Alternative names
    • FKSG80 antibody
    • FKSG80 protein antibody
    • G protein coupled receptor 104 antibody
    • G protein coupled receptor 81 antibody
    • G protein coupled receptor GPR81 antibody
    • G-protein coupled receptor 104 antibody
    • G-protein coupled receptor 81 antibody
    • GPR 104 antibody
    • GPR 81 antibody
    • GPR104 antibody
    • GPR81 antibody
    • GPR81_HUMAN antibody
    • HCA1 antibody
    • HCAR1 antibody
    • hydroxy-carboxylic acid receptor 1 antibody
    • Hydroxycarboxylic acid receptor 1 antibody
    • LACR1 antibody
    • lactate receptor 1 antibody
    • Putative chemokine receptor antibody
    • T cell activation G protein coupled receptor antibody
    • TA GPCR antibody
    see all

Images

  • Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hourAn additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.

References

This product has been referenced in:
  • Wagner W  et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . Read more (PubMed: 26208712) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106942.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up