Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR87
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human GPCR GPR87.

  • Positive control
    • Extracts from HUVEC cells and HT-29 cells. HUVEC cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Receptor for lysophosphatidic acid (LPA). Necessary for p53/TP53-dependent survival in response to DNA damage.
  • Tissue specificity
    Expressed in placenta and prostate. Weaker expression in thymus. Not expressed in thalamus, hippocampus, pons or cerebellum. Overexpressed in squamous cell carcinoma of the lung.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG 78 antibody
    • FKSG78 antibody
    • G protein coupled receptor 87 antibody
    • G protein coupled receptor 95 antibody
    • G-protein coupled receptor 87 antibody
    • G-protein coupled receptor 95 antibody
    • GPCR87 antibody
    • GPR 87 antibody
    • GPR 95 antibody
    • Gpr87 antibody
    • GPR87_HUMAN antibody
    • GPR95 antibody
    • hGPCR87 antibody
    • KPG 002 antibody
    • KPG_002 antibody
    • MGC131898 antibody
    • Orphan GPCR 87 antibody
    • Probable G protein coupled receptor 87 antibody
    see all

Images

  • All lanes : Anti-GPCR GPR87 antibody (ab77517) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,25 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of GPCR GPR87 in HUVEC cells using ab77517, at 1/500 dilution, in the absence or presence of immunising peptide.

References

This product has been referenced in:
  • Zhang X  et al. G Protein-Coupled Receptor 87 (GPR87) Promotes Cell Proliferation in Human Bladder Cancer Cells. Int J Mol Sci 16:24319-31 (2015). WB . Read more (PubMed: 26473854) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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