Overview

  • Product nameAnti-GPCR GPRC5B antibody
    See all GPCR GPRC5B primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPRC5B
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human GPCR GPRC5B.

  • Positive control
    • Extracts from HUVEC, 293 and Jurkat cells. MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionUnknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
  • Tissue specificityExpression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to PubMed:10945465, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
  • Cellular localizationCell membrane. Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 69G12.1 antibody
    • A-69G12.1 antibody
    • G protein coupled receptor family C group 1 member B antibody
    • G protein coupled receptor family C group 5 member B antibody
    • G-protein coupled receptor family C group 5 member B antibody
    • GPC5B_HUMAN antibody
    • Gprc5b antibody
    • LOC51704 antibody
    • RAIG-2 antibody
    • RAIG2 antibody
    • Retinoic acid responsive gene protein antibody
    • Retinoic acid-induced gene 2 protein antibody
    see all

Anti-GPCR GPRC5B antibody images

  • ab75427 staining MCF7 cells at 1/500 dilution, in the absence and presence of the immunising peptide
  • All lanes : Anti-GPCR GPRC5B antibody (ab75427) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from 293 cells
    Lane 3 : extracts from Jurkat cells
    Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GPCR GPRC5B antibody (ab75427)

ab75427 has not yet been referenced specifically in any publications.

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