Recombinant
RabMAb

Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

Overview

  • Product name
    Anti-GPCR LGR6 antibody [EPR6874]
    See all GPCR LGR6 primary antibodies
  • Description
    Rabbit monoclonal [EPR6874] to GPCR LGR6
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GPCR LGR6 aa 850-950 (intracellular). The exact sequence is proprietary.
    Database link: Q9HBX8

  • Positive control
    • Human uterus, ovary, HUVEC, Human adrenal gland and HeLa lysates; Human adrenal gland tissue; HUVEC cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6874
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

      Lane 1 : Human uterus lysate
      Lane 2 : Human ovary lysate
      Lane 3 : HUVEC lysate
      Lane 4 : Human adrenal gland lysate
      Lane 5 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 104 kDa
    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling GPCR LGR6 with purified ab126747 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Overlay histogram showing LoVo cells stained with ab126747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126747, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    ab126747 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your reply.

    You could make an antibody against Zebrafish lgr6 but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more i...

    Read More

    Thank you for supplying that information.

    I aligned the zebrafish sequence with the human sequence and there was only a 59% level of homology, between the 2. I also aligned the actual immunogen sequence used for ab126747, and the level of homo...

    Read More

    Thank you for contacting Abcam.

    I would be happy to help determine the level of homolgoy between our lgr6 antibodies and Zebrafish. However I am unable to locate the Zebrafish lgr6 sequence, and so I was wandering if you would be able to provi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up