Overview

  • Product nameAnti-GPCR LGR7 antibody
    See all GPCR LGR7 primary antibodies
  • Description
    Rabbit polyclonal to GPCR LGR7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chimpanzee, Monkey
    Predicted to work with: Chicken, Cow, Dog, Rhesus monkey, Opossum, Orangutan
  • Immunogen

    Synthetic peptide: VDDCGNQADE DNCGDNNGWS LQFDKYFASY YKMTSQYPFE AETPECLVGS V , corresponding to amino acids 50-100 of Human GPCR LGR7

  • Positive control
    • Human, mouse and rat heart lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • FunctionReceptor for relaxins. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. Binding of the ligand may also activate a tyrosine kinase pathway that inhibits the activity of a phosphodiesterase that degrades cAMP.
  • Tissue specificityExpressed in the brain, kidney, testis, placenta, uterus, ovary, adrenal, prostate, skin and heart. Not detected in spleen.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    Contains 1 LDL-receptor class A domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gm1018 antibody
    • Leucine rich repeat containing G protein coupled receptor 7 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 7 antibody
    • LGR 7 antibody
    • LGR7 antibody
    • LGR7.1 antibody
    • LGR7.10 antibody
    • LGR7.2 antibody
    • MGC138347 antibody
    • MGC142177 antibody
    • Relaxin family peptide receptor 1 antibody
    • Relaxin receptor 1 antibody
    • Relaxin/insulin like family peptide receptor 1 antibody
    • RXFP 1 antibody
    • RXFP1 antibody
    • RXFP1_HUMAN antibody
    see all

Anti-GPCR LGR7 antibody images

  • Lanes 1 - 2 : Anti-GPCR LGR7 antibody (ab72159) at 0.5 µg/ml
    Lane 3 : Anti-GPCR LGR7 antibody (ab72159) at 1 µg/ml
    Lane 4 : Anti-GPCR LGR7 antibody (ab72159) at 2 µg/ml

    Lane 1 : human heart lysate
    Lane 2 : human heart lysate with immunizing peptide
    Lane 3 : mouse heart lysate
    Lane 4 : rat heart lysate


    Predicted band size : 87 kDa
    Observed band size : 87 kDa

References for Anti-GPCR LGR7 antibody (ab72159)

This product has been referenced in:
  • Dehghan F  et al. Changes in Knee Laxity and Relaxin Receptor Isoforms Expression (RXFP1/RXFP2) in the Knee throughout Estrous Cycle Phases in Rodents. PLoS One 11:e0160984 (2016). WB ; Rat . Read more (PubMed: 27513858) »
  • Dehghan F  et al. Testosterone reduces knee passive range of motion and expression of relaxin receptor isoforms via 5a-dihydrotestosterone and androgen receptor binding. Int J Mol Sci 15:4619-34 (2014). WB ; Rat . Read more (PubMed: 24642882) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - 0.25 % Triton
Fixative Paraformaldehyde
Username

Dr. Cheryl Heesch

Verified customer

Submitted Oct 17 2013

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for contacting us and for providing the details of your protocol. I would expect the protocol you provided to give good signal on rat uterus lysates in WB. For our quality investigation, can you confirm that there were no bands at all on the ...

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