Human Predicted to work with:
Chimpanzee, Monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide within Human GPCR MRGX2 (extracellular). The exact sequence is proprietary. synthetic 14 amino acid peptide from 2nd extracellular domain of human GPCR MRGX2. NP_473371.1. Database link: Q96LB1
Human spinal cord (dorsal root ganglion) tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled.
Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion.
Belongs to the G-protein coupled receptor 1 family. Mas subfamily.