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Rabbit polyclonal to GPCR RDC1
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GPCR RDC1.
(Peptide available as
This antibody gave a positive result with human brain, testis and heart tissue lysates. Human kidney is a suitable positive control for IHC.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with
Human GPCR RDC1 peptide (ab38088).
GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
atypical chemokine receptor 3 antibody
C X C chemokine receptor type 7 antibody
Western blot - GPCR RDC1 antibody (ab38089)
All lanes :
Anti-GPCR RDC1 antibody (ab38089) at 1/250 dilution
Lane 1 :
Human Brain Tissue Lysate
Lane 2 :
Human Testis Tissue Lysate
Lane 3 :
Human Liver Tissue Lysate
Lane 4 :
Human Heart Tissue Lysate
Lysates/proteins at 10 µg per lane.
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size :
Observed band size :
52 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GPCR RDC1 antibody (ab38089) This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab38089 staining pancreas (human and mouse) kidney (mouse and rat) and testis (rat and human) tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"