Overview

  • Product nameAnti-GPCR TGR5 antibody
    See all GPCR TGR5 primary antibodies
  • Description
    Rabbit polyclonal to GPCR TGR5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GPCR TGR5.

  • Positive control
    • This antibody gave a positive signal in HepG2 and Caco2 whole cell lysates. IF/ICC: HCT116 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionReceptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BG 37 antibody
    • BG37 antibody
    • G protein coupled bile acid receptor 1 antibody
    • G protein coupled bile acid receptor BG 37 antibody
    • G protein coupled bile acid receptor BG37 antibody
    • G-protein coupled bile acid receptor 1 antibody
    • G-protein coupled receptor GPCR19 antibody
    • GPBAR 1 antibody
    • GPBAR_HUMAN antibody
    • GPBAR1 antibody
    • GPCR 19 antibody
    • GPCR antibody
    • GPCR19 antibody
    • GPR 131 antibody
    • GPR131 antibody
    • hBG 37 antibody
    • hBG37 antibody
    • hGPCR 19 antibody
    • hGPCR19 antibody
    • M BAR antibody
    • M-BAR antibody
    • Membrane bile acid receptor antibody
    • Membrane type receptor for bile acids antibody
    • Membrane-type receptor for bile acids antibody
    • MGC40597 antibody
    • TGR 5 antibody
    • TGR5 antibody
    see all

Anti-GPCR TGR5 antibody images

  • ICC/IF image of ab114081 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab114081, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GPCR TGR5 antibody (ab114081) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa,71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
    The predicted molecular weight of GPCR TGR5 is 35 kDa (SwissProt), however we expect to observe a banding pattern at 33 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References for Anti-GPCR TGR5 antibody (ab114081)

ab114081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"