The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 35 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
Belongs to the G-protein coupled receptor 1 family.
ab117414, at 10 ug/ml, staining GPCR TGR5 in Formalin-fixed, Paraffin-embedded Human Colon tissue by Immunohistochemistryfollowed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Western blot - Anti-GPCR TGR5 antibody (ab117414)
All lanes : Anti-GPCR TGR5 antibody (ab117414) at 3 µg/ml
Lane 1 : Human kidney lysate Lane 2 : Human kidney lysate with immunizing peptide