The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 2 - 4 µg/ml. Detects bands of approximately 33 and 50 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
Tissue specificityUbiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab71988 at 5µg/ml staining GPCR TGR5 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin embedded tissue section). The top left image show staining with the Isotype control antibody, the bottom left and right image show staining with ab71988.
References for Anti-GPCR TGR5 antibody (ab71988)
This product has been referenced in:
Ockenga J et al. Plasma bile acids are associated with energy expenditure and thyroid function in humans. J Clin Endocrinol Metab97:535-42 (2012).
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