Overview

  • Product nameAnti-GPCR TGR5 antibody
    See all GPCR TGR5 primary antibodies
  • Description
    Rabbit polyclonal to GPCR TGR5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey
  • Immunogen

    A synthetic peptide (LWGRASRDSPGPSIAYHPSSQ) corresponding to amino acids 302-322 of human BG37/TGR5 was used as the immunogen, GenBank no NP_733800.1.

  • Positive control
    • Human kidney lysate, human colon tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects bands of approximately 33 and 50 kDa (predicted molecular weight: 37 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
    • Tissue specificityUbiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BG 37 antibody
      • BG37 antibody
      • G protein coupled bile acid receptor 1 antibody
      • G protein coupled bile acid receptor BG 37 antibody
      • G protein coupled bile acid receptor BG37 antibody
      • G-protein coupled bile acid receptor 1 antibody
      • G-protein coupled receptor GPCR19 antibody
      • GPBAR 1 antibody
      • GPBAR_HUMAN antibody
      • GPBAR1 antibody
      • GPCR 19 antibody
      • GPCR antibody
      • GPCR19 antibody
      • GPR 131 antibody
      • GPR131 antibody
      • hBG 37 antibody
      • hBG37 antibody
      • hGPCR 19 antibody
      • hGPCR19 antibody
      • M BAR antibody
      • M-BAR antibody
      • Membrane bile acid receptor antibody
      • Membrane type receptor for bile acids antibody
      • Membrane-type receptor for bile acids antibody
      • MGC40597 antibody
      • TGR 5 antibody
      • TGR5 antibody
      see all

    Anti-GPCR TGR5 antibody images

    • All lanes : Anti-GPCR TGR5 antibody (ab71988) at 3 µg/ml

      Lane 1 : human kidney lysate
      Lane 2 : human kidney lysate with immunising peptide


      Predicted band size : 37 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • ab71988 at 5µg/ml staining GPCR TGR5 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin embedded tissue section). The top left image show staining with the Isotype control antibody, the bottom left and right image show staining with ab71988.

    References for Anti-GPCR TGR5 antibody (ab71988)

    This product has been referenced in:
    • Ockenga J  et al. Plasma bile acids are associated with energy expenditure and thyroid function in humans. J Clin Endocrinol Metab 97:535-42 (2012). IHC ; Human . Read more (PubMed: 22162464) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"