Overview

  • Product nameAnti-GPCR TGR5 antibody
    See all GPCR TGR5 primary antibodies
  • Description
    Rabbit polyclonal to GPCR TGR5
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 250 and 300 of human GPCR TGR5 (NP_733800.1).

  • Positive control
    • Human, mouse and rat spleen lysates.
  • General notesGPCR is multi pass membrane protein, customers may try heating the lysates for 15-20 minutes at 60-70C instead of boiling the samples. Non fat dry milk as blocking agent seems effective than BSA with current lot. Please contact technical support team for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 23341498

Target

  • FunctionReceptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BG 37 antibody
    • BG37 antibody
    • G protein coupled bile acid receptor 1 antibody
    • G protein coupled bile acid receptor BG 37 antibody
    • G protein coupled bile acid receptor BG37 antibody
    • G-protein coupled bile acid receptor 1 antibody
    • G-protein coupled receptor GPCR19 antibody
    • GPBAR 1 antibody
    • GPBAR_HUMAN antibody
    • GPBAR1 antibody
    • GPCR 19 antibody
    • GPCR antibody
    • GPCR19 antibody
    • GPR 131 antibody
    • GPR131 antibody
    • hBG 37 antibody
    • hBG37 antibody
    • hGPCR 19 antibody
    • hGPCR19 antibody
    • M BAR antibody
    • M-BAR antibody
    • Membrane bile acid receptor antibody
    • Membrane type receptor for bile acids antibody
    • Membrane-type receptor for bile acids antibody
    • MGC40597 antibody
    • TGR 5 antibody
    • TGR5 antibody
    see all

Anti-GPCR TGR5 antibody images

  • Lane 1 :
    Lane 2 : Anti-GPCR TGR5 antibody (ab72608) at 1/1000 dilution
    Lanes 3 - 4 : Anti-GPCR TGR5 antibody (ab72608) at 1/5000 dilution

    Lane 1 : Pre-immune serum
    Lane 2 : Human spleen lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat spleen lysate


    Predicted band size : 35 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GPCR TGR5 antibody (ab72608)

This product has been referenced in:
  • Renga B  et al. Cystathionine ?-lyase, a H2S-generating enzyme, is a GPBAR1-regulated gene and contributes to vasodilation caused by secondary bile acids. Am J Physiol Heart Circ Physiol 309:H114-26 (2015). Read more (PubMed: 25934094) »
  • Domingue JC  et al. HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport. Physiol Rep 2:N/A (2014). Human . Read more (PubMed: 25263207) »

See all 6 Publications for this product

Product Wall

We expect that glycosylation will apeear as a smear on the blot, rather than distinct bands, so I am skeptical that you are detecting TGR5. In addition, you should be seeing more signal in the membrane fraction, and not an identical banding pattern in ...

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L’anticorps ab72608 n’a pas été purifié et est un antisérum entier. La concentration n’a donc pas été déterminé par contre nous estimons que la concentration sera de 1-10 mg/ml.

Thank you for your enquiry regarding ab72608 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

The protocol looks absolutely fine to me, I would...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting Abcam about ab72608.

We are unable to provide the peptide that was used to generate ab72608 (it was all used up for development and testing of the antibody). However, you should be able to have it readily synthesized ...

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Thank you for your email.

I am sorry to hear that the antibody did not work. I can help you with troubleshooting so that you only have to repeat the experiment once; although there was a typos error in image but it should not have affected the...

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Thank you for your enquiry.

I can confirm that ab72608 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datashe...

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Thank you for your enquiry.

I can confirm that ab72608 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datashe...

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Thank you for contacting Abcam.

I am sorry that you were experiencing some difficulties with ab72608 in western blot. If the protocol variations that we talked about do not prove to be successful, then please let me know and I will be happy to...

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Thank you very much for your reply.

I will be happy to send a fresh vial to try. Could you let me know the PO or order number once your purchasing agent has found this information? I will need it to process the replacement.

Please l...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"