Overview

  • Product name
  • Description
    Rabbit polyclonal to GPLD1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human GPLD1.

    (Peptide available as ab92422.)

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Plasma Total Protein Preparations.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
    • Sequence similarities
      Belongs to the GPLD1 family.
      Contains 7 FG-GAP repeats.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glycoprotein phospholipase D antibody
      • Glycosyl-phosphatidylinositol-specific phospholipase D antibody
      • GPI-PLD antibody
      • GPI-specific phospholipase D antibody
      • GPIPLD antibody
      • GPIPLDM antibody
      • GPLD1 antibody
      • MGC22590 antibody
      • PHLD_HUMAN antibody
      • Phosphatidylinositol glycan specific phospholipase D antibody
      • Phosphatidylinositol-glycan-specific phospholipase D antibody
      • PI-G PLD antibody
      • PIGPLD antibody
      • PIGPLD1 antibody
      see all

    Images

    • All lanes : Anti-GPLD1 antibody (ab82330) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Mouse Plasma Total Protein Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 92 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

    References

    ab82330 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82330.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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