Overview

  • Product nameAnti-GPM6B antibody
    See all GPM6B primary antibodies
  • Description
    Rabbit polyclonal to GPM6B
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human GPM6B.

  • Positive control
    • This antibody gave a positive signal in Mouse cerebellum tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab92988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • FunctionMay be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.
  • Tissue specificityNeurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia.
  • Sequence similaritiesBelongs to the myelin proteolipid protein family.
  • Cellular localizationCell membrane. Colocalizes with SERT at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • glycoprotein M6B antibody
    • GPM6B antibody
    • GPM6B_HUMAN antibody
    • M6b antibody
    • Neuronal membrane glycoprotein M6-b antibody
    • Neuronal membrane glycoprotein M6b antibody
    • protolipid M6B antibody
    • rhombex 29 antibody
    see all

Anti-GPM6B antibody images

  • Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GPM6B antibody (ab92988)

ab92988 has not yet been referenced specifically in any publications.

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