Overview

  • Product name
  • Description
    Rabbit polyclonal to GPM6B
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human GPM6B.

  • Positive control
    • This antibody gave a positive signal in Mouse cerebellum tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab92988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Function
    May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.
  • Tissue specificity
    Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia.
  • Sequence similarities
    Belongs to the myelin proteolipid protein family.
  • Cellular localization
    Cell membrane. Colocalizes with SERT at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • glycoprotein M6B antibody
    • GPM6B antibody
    • GPM6B_HUMAN antibody
    • M6b antibody
    • Neuronal membrane glycoprotein M6-b antibody
    • Neuronal membrane glycoprotein M6b antibody
    • protolipid M6B antibody
    • rhombex 29 antibody
    see all

Anti-GPM6B antibody images

  • Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GPM6B antibody (ab92988)

This product has been referenced in:
  • Choi KM  et al. Distribution of the Immunoreactivity for Glycoprotein M6B in the Neurogenic Niche and Reactive Glia in the Injury Penumbra Following Traumatic Brain Injury in Mice. Exp Neurobiol 22:277-82 (2013). IHC . Read more (PubMed: 24465143) »

See 1 Publication for this product

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