Overview

  • Product nameAnti-GPNMB antibody
    See all GPNMB primary antibodies
  • Description
    Mouse monoclonal to GPNMB
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: RCQKEDANGN IVYEKNCRNE AGLSADPYVY NWTAWSEDSD GENGTGQSHH NVFPDGKPFP HHPGWRRWNF IYVFHTLGQY FQKLGRCSVR VSVNTANVTL , corresponding to amino acids 104-204 of Human GPNMB

Properties

Applications

Our Abpromise guarantee covers the use of ab56584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: Use at an assay dependent dilution (PMID 20133820).
    WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCould be a melanogenic enzyme.
    • Tissue specificityNot restricted to the melanocytic lineage.
    • Sequence similaritiesBelongs to the PMEL/NMB family.
      Contains 1 PKD domain.
    • Developmental stageExpression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
    • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glycoprotein (transmembrane) nmb antibody
      • Glycoprotein nmb antibody
      • Glycoprotein nmb like protein antibody
      • Gpnmb antibody
      • GPNMB_HUMAN antibody
      • HGFIN antibody
      • NMB antibody
      • Osteoactivin antibody
      • Transmembrane glycoprotein antibody
      • Transmembrane glycoprotein HGFIN antibody
      • Transmembrane glycoprotein NMB antibody
      see all

    Anti-GPNMB antibody images

    • Western blot against tagged recombinant protein immunogen using ab56584 GPNMB antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-GPNMB antibody (ab56584)

    This product has been referenced in:
    • Tyburczy ME  et al. Novel Proteins Regulated by mTOR in Subependymal Giant Cell Astrocytomas of Patients with Tuberous Sclerosis Complex and New Therapeutic Implications. Am J Pathol 176:1878-90 (2010). IHC-P ; Human . Read more (PubMed: 20133820) »

    See 1 Publication for this product

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