Overview

  • Product nameAnti-GPR161 antibody
    See all GPR161 primary antibodies
  • Description
    Rabbit polyclonal to GPR161
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MSLNSSLSCR KELSNLTEEE GGEGGVIITQ FIAIIVITIF VCLGNLVIVV, corresponding to N terminal amino acids 1-50 of Human GPR161

  • Positive control
    • HepG2 cell lysate or human kidney tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-GPR161 antibody images

  • ICC/IF image of ab58679 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58679 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GPR161 antibody (ab58679) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPR161 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-GPR161 antibody (ab58679)

This product has been referenced in:
  • Feigin ME  et al. G-protein-coupled receptor GPR161 is overexpressed in breast cancer and is a promoter of cell proliferation and invasion. Proc Natl Acad Sci U S A 111:4191-6 (2014). Human . Read more (PubMed: 24599592) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Mouse Cell (NIH3T3 cells)
Specification NIH3T3 cells
Permeabilization Yes - 0.2% Tween 20
Fixative Paraformaldehyde
Username

Dr. Xuecai Ge

Verified customer

Submitted Aug 09 2013

Thank you very much for your interest in ab58679.

To our knowledge, ab58679 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab58679 now, test it in Flow Cytometryand submit feedback to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"