Overview

  • Product name
    Anti-GPR180 antibody
  • Description
    Rabbit polyclonal to GPR180
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 126-155 of Human GPR180.

  • Positive control
    • WiDr, Y79, 293, A2058 and A375 cell lysates; Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab103453 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-GPR180 antibody (ab103453) at 1/100 dilution

    Lane 1 : WiDr cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : A2058 cell lysate
    Lane 5 : A375 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 49 kDa
  • ab103453, at 1/50 dilution, staining GPR180 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

ab103453 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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