Overview

  • Product name
  • Description
    Goat polyclonal to GPR2/CCR10
  • Specificity
    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • Tested applications
    Suitable for: Flow Cyt, ELISA, ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    KPTEQVSWGLYSGYDEEAYSVGPLPELC

    , corresponding to amino acids 4-31 of Mouse CCR10.

  • Positive control
    • Mouse lymph nodes or small intestine.
  • General notes


    Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells.
ELISA 1/100000.
ICC 1/500.
WB 1/1000.
IHC-P 1/250.

Target

  • Function
    Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.
  • Tissue specificity
    Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C chemokine receptor type 10 antibody
    • C-C chemokine receptor type 10 antibody
    • C-C CKR-10 antibody
    • CC chemokine receptor 10 antibody
    • CC CKR 10 antibody
    • CC-CKR-10 antibody
    • CCR-10 antibody
    • CCR10 antibody
    • CCR10_HUMAN antibody
    • Chemokine (C C motif) receptor 10 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 2 antibody
    • GPR2 antibody
    see all

Images

  • Immunohistochemistry using ab1661 on a section of mouse small intestine

References

This product has been referenced in:
  • Hu K  et al. CCL19 and CCL28 augment mucosal and systemic immune responses to HIV-1 gp140 by mobilizing responsive immunocytes into secondary lymph nodes and mucosal tissue. J Immunol 191:1935-47 (2013). Read more (PubMed: 23858028) »
  • Shankar SP  et al. RGS16 attenuates pulmonary Th2/Th17 inflammatory responses. J Immunol 188:6347-56 (2012). ICC ; Mouse . Read more (PubMed: 22593615) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
I-Block (Casein-based blocking reagent, ABI) as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Miss. A Uhmann

Verified customer

Submitted Sep 29 2010

Thank you for your enquiry. The application of flow cytometry/FACS was not originally tested for this antibody. A customer purchased this antibody and wrote a review on the website stating that the antibody was used successfully for flow cytometry ...

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Thank you for your e-mail. I would recommend the same steps as for frozen sections (long incubations, presence of triton in your buffers, checking the amplification kits work for other antibodies as these may go off). Finally you may wish to try a ...

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I'm sorry to hear you are having problems with ab1661. I would like to suggest some modifications to your protocol to help you detect CCR10 in your samples. -When fixing frozen sections, try ice-cold methanol/acetone for 10min. -check you permeabi...

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Yes, ab7004 is an appropriate secondary antibody to use with ab1661. When you place your order, you can make your payment with a Mastercard or Visa credit card or you can use a Purchase Order number. An invoice will be mailed to you either the day o...

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To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a rewa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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