Overview

  • Product name
  • Description
    Goat polyclonal to GPR2/CCR10
  • Specificity
    Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISA, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    VSWGHYSGDEEDAYSAEPLPELC

    , corresponding to amino acids 9-31 of Human CCR10.

  • General notes


    Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    10 mM KHPO4, 140 mM NaCl, pH 7.2.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Can be blocked with GPR2/CCR10 peptide (ab182115).
ICC/IF Use at an assay dependent concentration.
ELISA 1/75000.
ICC 1/1000.
Flow Cyt 1/25.

See Eksteen et al; Von Luttichau et al; Notohamiprodjo et al; Hirata et al and Hislop et al.



IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval at pH 6.0 or pH 8.0; steam for 20 min followed by cooling for 20 min.

Target

  • Function
    Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.
  • Tissue specificity
    Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C chemokine receptor type 10 antibody
    • C-C chemokine receptor type 10 antibody
    • C-C CKR-10 antibody
    • CC chemokine receptor 10 antibody
    • CC CKR 10 antibody
    • CC-CKR-10 antibody
    • CCR-10 antibody
    • CCR10 antibody
    • CCR10_HUMAN antibody
    • Chemokine (C C motif) receptor 10 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 2 antibody
    • GPR2 antibody
    see all

Images

  • Immunohistochemistry on paraffin sections of human spleen.
  • After retroviral transduction, CCR10-B16 cells were lysed, and CCR10 was detected by Western blotting after gel electrophoresis. A single band representing CCR10 was detected at 42 kD.
  • ab3904, at a dilution of 1/250, staining CCR10 in human tonsil section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, staining GPR2/CCR10 with ab3904.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A biotinylated rabbit anti-goat polyclonal IgG (10 µg/ml) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Pinto S  et al. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer 14:118 (2014). ICC . Read more (PubMed: 24559071) »
  • Chamberlain G  et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt ; Human, Mouse . Read more (PubMed: 18698345) »

See all 7 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (human serum samples)
Specification
human serum samples
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (keratinocytes)
Specification
keratinocytes
Preparation
Cell harvesting/tissue preparation method: cells were isolated from skin biopsie followed by cell culture
Sample buffer: PBS/10% human IgG
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,1% Tritonx-100/PBS
Gating Strategy
no gate
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocytes)
Loading amount
30 µg
Specification
Keratinocytes
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2013

Thank you for your reply. A quotation has been sent to you in a separate email, the proforma reference number is : *****. DISCOUNT CODE for ab1667 to be tested in Flow Cytometry : ******** Expiration date: DD MM YYYY I am very pleased to hear you would...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Keratinocytes)
Specification
Keratinocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 03 2012

Thank you for your enquiry. I am sorry we have no conjugated antibodies available raised against CCR10. There are two primary antibodies that would be suitable for your application, ab3904 and ab1658. You could perhaps use a conjugated secondary with o...

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Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. I have read your customer's technical questionnaire and I have a few comments. Please can you ask your customer to comment on the...

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Thank you for your enquiry. I have obtained a Western blot image and added it to the datasheet for the antibody. I have included a link to the datasheet at the bottom of this email. This link is from the following paper: Murakami T, Cardones AR,...

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Thank you for your enquiry. The starting dilution recommended for this antibody is 1/250. Other than that, you should be able to do a standard Western blot protocol (such as the one listed on our site at http://www.abcam.com/index.html?pageconfig=v...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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