Overview

  • Product name
  • Description
    Rabbit polyclonal to GPRC5C
  • Specificity
    Detects endogenous levels of total GPRC5C protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal region of Human GPRC5C

  • Positive control
    • Cos-7 cell extract RAW264.7 cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/5000.

Target

  • Function
    This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
  • Tissue specificity
    Expression is highest in the periphery, particularly in the stomach, but also in the kidney, liver, pancreas, and prostate. In brain, levels of expression are generally lower than in the periphery, with the exception of cerebellum, spinal cord, and dorsal root ganglia (DRG).
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor family C group 5 member C antibody
    • G-protein coupled receptor family C group 5 member C antibody
    • GPC5C_HUMAN antibody
    • GPCR5C antibody
    • Gprc5c antibody
    • MGC131820 antibody
    • Orphan G protein coupled receptor antibody
    • RAIG-3 antibody
    • RAIG3 antibody
    • Retinoic acid responsive gene protein antibody
    • Retinoic acid-induced gene 3 protein antibody
    see all

Images

  • All lanes : Anti-GPRC5C antibody (ab75499) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : Cos-7 cell extract
    Lane 3 : RAW264.7 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

References

ab75499 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75499.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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