Anti-GPSM2 antibody (ab84571)

Overview

  • Product nameAnti-GPSM2 antibodySee all GPSM2 primary antibodies ...
  • Description
    Rabbit polyclonal to GPSM2
  • Tested applicationsICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (YFYLHDYAKA LEYHHHDLTL ARTIGDQLGE AKASGNLGNT LKVLGNFDEA) of Human GPSM2 (NP_037428).

  • Positive control
    • Human fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
  • Sequence similaritiesBelongs to the GPSM family.
    Contains 4 GoLoco domains.
    Contains 8 TPR repeats.
  • Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNB82 antibody
    • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
    • G protein signalling modulator 2 antibody
    • G-protein-signaling modulator 2 antibody
    • Gpsm2 antibody
    • GPSM2_HUMAN antibody
    • HGNC:29501 antibody
    • LGN antibody
    • LGN protein antibody
    • Mosaic protein LGN antibody
    • Pins antibody
    see all

Anti-GPSM2 antibody images

  • ab84571 staining GPSM2 in Dog kidney cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C.

    See Abreview

  • ab84571 at 5 µg/ml staining GPSM2 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-GPSM2 antibody (ab84571) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab84571 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab84571 at 5 µg/ml staining GPSM2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-GPSM2 antibody (ab84571)

ab84571 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3%
Sample Dog Cell (kidney)
Specification kidney
Permeabilization Yes - Triton 0,5%
Fixative Paraformaldehyde
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Dr. Inma Banon

Verified customer

Submitted Oct 11 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your response.

I would expect ab31850 to give clearer results in WB, even once the many isoforms are taken into consideration. I would be happy to offer you a free of charge replacement, credit, or refund for this antibody as we...

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For the Spastin antibody ab31850, could you please provide an image of your blot or let me know what size bands you are observing? Spastin has several known isoforms that could account for multiple bands between 54-6...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse Embryo)
Specification Mouse Embryo
Fixative Paraformaldehyde
Permeabilization Yes - Saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
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Abcam user community

Verified customer

Submitted Mar 14 2012

Thank you for your phone call.

DISCOUNT CODE: xxx for ab84571
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I am very pleased to hear you would like to accept our offer and test ab84571, ab111022 in mouse. Each cod...

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