• Product name
  • Description
    Rabbit polyclonal to GPT2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GPT2.

  • Positive control
    • This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates.



Our Abpromise guarantee covers the use of ab101876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).


  • Function
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
  • Tissue specificity
    Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
  • Pathway
    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similarities
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT2 antibody
    • Alanine aminotransferase 2 antibody
    • ALAT2_HUMAN antibody
    • ALT2 antibody
    • Glutamate pyruvate transaminase 2 antibody
    • Glutamic alanine transaminase 2 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) 2 antibody
    • Glutamic pyruvic transaminase 2 antibody
    • Glutamic--alanine transaminase 2 antibody
    • Glutamic--pyruvic transaminase 2 antibody
    • GPT 2 antibody
    • gpt2 antibody
    see all


  • All lanes : Anti-GPT2 antibody (ab101876) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes
  • ICC/IF image of ab101876 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101876, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.


ab101876 has not yet been referenced specifically in any publications.

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