Overview

  • Product nameAnti-GPT2 antibody
    See all GPT2 primary antibodies
  • Description
    Rabbit polyclonal to GPT2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GPT2.

  • Positive control
    • This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab101876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).

Target

  • FunctionCatalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
  • Tissue specificityExpressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
  • PathwayAmino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT2 antibody
    • Alanine aminotransferase 2 antibody
    • ALAT2_HUMAN antibody
    • ALT2 antibody
    • Glutamate pyruvate transaminase 2 antibody
    • Glutamic alanine transaminase 2 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) 2 antibody
    • Glutamic pyruvic transaminase 2 antibody
    • Glutamic--alanine transaminase 2 antibody
    • Glutamic--pyruvic transaminase 2 antibody
    • GPT 2 antibody
    • gpt2 antibody
    see all

Anti-GPT2 antibody images

  • All lanes : Anti-GPT2 antibody (ab101876) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab101876 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101876, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-GPT2 antibody (ab101876)

ab101876 has not yet been referenced specifically in any publications.

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