Overview

  • Product nameAnti-GRAF antibody
    See all GRAF primary antibodies
  • Description
    Goat polyclonal to GRAF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KTGLIPENYVEFL

    , corresponding to C terminal amino acids 802-814 of Human GRAF.

  • Positive control
    • Heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).

Target

  • FunctionGTPase-activating protein for RHOA and CDC42.
  • Involvement in diseaseDefects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
  • Sequence similaritiesContains 1 PH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • arhgap26 antibody
    • FLJ42530 antibody
    • GRAF antibody
    • GRAF1 antibody
    • GTPase regulator associated with focal adhesion kinase antibody
    • GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody
    • KIAA0621 antibody
    • oligophrenin 1 like protein antibody
    • Oligophrenin-1-like protein antibody
    • oligophrenin1like protein antibody
    • OPHN1L antibody
    • OPHN1L1 antibody
    • RHG26_HUMAN antibody
    • Rho GTPase activating protein 26 antibody
    • Rho GTPase activating protein26 antibody
    • Rho GTPase-activating protein 26 antibody
    • Rho-type GTPase-activating protein 26 antibody
    see all

Anti-GRAF antibody images

  • All lanes : Anti-GRAF antibody (ab15916) at 0.3 µg/ml

    Lane 1 : (A) Human Heart lysate
    Lane 2 : (B) Human Heart lysate with immunizing peptide

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 94 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GRAF antibody (ab15916)

ab15916 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"