Overview

  • Product nameAnti-GRAF antibody
    See all GRAF primary antibodies
  • Description
    Mouse polyclonal to GRAF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human GRAF (AAH68555.1, amino acids 1-759)

  • Positive control
    • GRAF transfected 293T cell lysate, HepG2 cell lysate and Human placenta tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: Isoform 2, 86 kDa; Isoform 1, 94 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGTPase-activating protein for RHOA and CDC42.
    • Involvement in diseaseDefects in ARHGAP26 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Chromosomal translocation t(5;11)(q31;q23) with MLL has been found in a JMML patient.
    • Sequence similaritiesContains 1 PH domain.
      Contains 1 Rho-GAP domain.
      Contains 1 SH3 domain.
    • Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton. Colocalizes with actin stress fibers and cortical actin structures.
    • Information by UniProt
    • Database links
    • Alternative names
      • arhgap26 antibody
      • FLJ42530 antibody
      • GRAF antibody
      • GRAF1 antibody
      • GTPase regulator associated with focal adhesion kinase antibody
      • GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody
      • KIAA0621 antibody
      • oligophrenin 1 like protein antibody
      • Oligophrenin-1-like protein antibody
      • oligophrenin1like protein antibody
      • OPHN1L antibody
      • OPHN1L1 antibody
      • RHG26_HUMAN antibody
      • Rho GTPase activating protein 26 antibody
      • Rho GTPase activating protein26 antibody
      • Rho GTPase-activating protein 26 antibody
      • Rho-type GTPase-activating protein 26 antibody
      see all

    Anti-GRAF antibody images

    • Anti-GRAF antibody (ab89783) at 1 µg/ml + human placenta tissue lysate at 50 µg

      Predicted band size : 86 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)
    • Anti-GRAF antibody (ab89783) at 1 µg/ml + HepG2 cell lysate at 50 µg

      Predicted band size : 86 kDa
      Observed band size : 86 kDa
    • All lanes : Anti-GRAF antibody (ab89783) at 1 µg/ml

      Lane 1 : GRAF transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 86 kDa
      Observed band size : 86 kDa

    References for Anti-GRAF antibody (ab89783)

    This product has been referenced in:
    • Cai B  et al. GRAF1 forms a complex with MICAL-L1 and EHD1 to cooperate in tubular recycling endosome vesiculation. Front Cell Dev Biol 2:22 (2014). Read more (PubMed: 25364729) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"