Anti-Grainyhead-like protein 1 homolog antibody (ab111582)
Key features and details
- Rabbit polyclonal to Grainyhead-like protein 1 homolog
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Grainyhead-like protein 1 homolog antibody -
Description
Rabbit polyclonal to Grainyhead-like protein 1 homolog -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-208 of Human Grainyhead-like protein 1 homolog.
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Positive control
- NT2D1, U-87 MG and MCF7 cells and whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111582 is purified by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Transcription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation (By similarity). Binds and transactivates the EN1 promoter. -
Tissue specificity
Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Highly expressed in placental JEG-3 cells and at very low levels in non-steroidogenic cells. No expression detected in adrenal NCI-H295A cells. -
Sequence similarities
Belongs to the grh/CP2 family. Grainyhead subfamily. -
Developmental stage
Expressed in fetal liver and brain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29841 Human
- Omim: 609786 Human
- SwissProt: Q9NZI5 Human
- Unigene: 418493 Human
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Alternative names
- Grainyhead-like protein 1 homolog antibody
- grhl1 antibody
- GRHL1_HUMAN antibody
see all
Images
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Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS-PAGE. -
ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111582 has not yet been referenced specifically in any publications.