Anti-Grainyhead-like protein 1 homolog antibody (ab77762)

Overview

  • Product name
    Anti-Grainyhead-like protein 1 homolog antibody
    See all Grainyhead-like protein 1 homolog primary antibodies
  • Description
    Rabbit polyclonal to Grainyhead-like protein 1 homolog
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Grainyhead-like protein 1 homolog.

    (Peptide available as ab88963.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: JEG-3; HepG2; HEK293; Jurkat.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Transcription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation (By similarity). Binds and transactivates the EN1 promoter.
  • Tissue specificity
    Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Highly expressed in placental JEG-3 cells and at very low levels in non-steroidogenic cells. No expression detected in adrenal NCI-H295A cells.
  • Sequence similarities
    Belongs to the grh/CP2 family. Grainyhead subfamily.
  • Developmental stage
    Expressed in fetal liver and brain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Grainyhead-like protein 1 homolog antibody
    • grhl1 antibody
    • GRHL1_HUMAN antibody
    • LBP32 antibody
    • Mammalian grainyhead antibody
    • MGR antibody
    • NH32 antibody
    • TFCP2L2 antibody
    • Transcription factor CP2-like 2 antibody
    • Transcription factor LBP-32 antibody
    see all

Images

  • All lanes : Anti-Grainyhead-like protein 1 homolog antibody (ab77762) at 1 µg/ml

    Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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