Anti-Gram Negative Endotoxin antibody [8.F.121] (ab31204)

Overview

  • Product nameAnti-Gram Negative Endotoxin antibody [8.F.121]
    See all Gram Negative Endotoxin primary antibodies
  • Description
    Mouse monoclonal [8.F.121] to Gram Negative Endotoxin
  • SpecificityAb31204 recognises E. coli J5 LPS.
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Tissue/ cell preparation: E. coli J5 whole cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesAb31204 is a purified IgG.
  • ClonalityMonoclonal
  • Clone number8.F.121
  • IsotypeIgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • RelevanceEndotoxins are part of the outer membrane of the cell wall of gram negative bacteria. Endotoxins are invariably associated with gram negative bacteria whether the organisms are pathogens or not. Although the term "endotoxin" is occasionally used to refer to any cell associated bacterial toxin, it is properly reserved to refer to the lipopolysaccharide complex associated with the outer membrane of gram-negative bacteria such as E. coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus, and other leading pathogens. The biological activity of endotoxins are associated with lipopolysaccharide (LPS). Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of gram negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses in an animal. Because it activates complement by the alternative (properdin) pathway, it is often part of the pathology of gram negative bacterial infections.
  • Cellular localizationCell Membrane
  • Alternative names
    • E coli LPS antibody

References for Anti-Gram Negative Endotoxin antibody [8.F.121] (ab31204)

ab31204 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"