For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
- Sequence similaritiesContains 1 GRAM domain.
- Cellular localizationMembrane.
Information by UniProt
- GRAM domain containing 1B antibody
- GRAM domain-containing protein 1B antibody
- Gramd1b antibody
Anti-GRAMD1B antibody images
Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1B antibody (ab121286)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab121286 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1B antibody (ab121286)
ab121286, at a 1/100 dilution, staining GRAMD1B in paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
Western blot - Anti-GRAMD1B antibody (ab121286)
All lanes : Anti-GRAMD1B antibody (ab121286) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
References for Anti-GRAMD1B antibody (ab121286)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"