Overview

  • Product name
  • Description
    Rabbit polyclonal to Granulin
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C-terminal region of human Granulin.

  • Positive control
    • Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab53880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 7 µg/reaction.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects bands varying from approximately 64-88 kDa depending on degree of protein glycosylation.(predicted molecular weight: 64 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
      Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
    • Tissue specificity
      In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
    • Involvement in disease
      Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
    • Sequence similarities
      Belongs to the granulin family.
    • Post-translational
      modifications
      Granulins are disulfide bridged.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acrogranin antibody
      • CLN11 antibody
      • GEP antibody
      • GP88 antibody
      • Granulin A antibody
      • Granulin B antibody
      • Granulin C antibody
      • Granulin D antibody
      • Granulin E antibody
      • Granulin epithelin antibody
      • Granulin F antibody
      • Granulin G antibody
      • Granulin-7 antibody
      • Granulins antibody
      • GRN antibody
      • GRN_HUMAN antibody
      • PC cell derived growth factor antibody
      • PCDGF antibody
      • PEPI antibody
      • PGRN antibody
      • Proepithelin antibody
      • Progranulin antibody
      see all

    Images

    • All lanes : Anti-Granulin antibody (ab53880) at 1 µg/ml

      Lane 1 : MDA-MB-231 cell lysate
      Lane 2 : PC-3 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT1080 cell lysate


      Predicted band size : 64 kDa
      Observed band size : 64 kDa

    References

    ab53880 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. I contacted the originator of ab53880 Granulin antibody who have kindly provided the following information. A BLAST search with the immunogen used for the antibody and the protein accession P28799 and below show 100%...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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