Overview

  • Product nameAnti-Granulin antibody
    See all Granulin primary antibodies
  • Description
    Mouse monoclonal to Granulin
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: SCEKEVVSAQ PATFLARSPH VGVKDVECGE GHFCHDNQTC CRDNRQGWAC CPYRQGVCCA DRRHCCPAGF RCAARGTKCL RREAPRWDAP LRDPALRQLL , corresponding to amino acids 494-594 of Human Granulin

Properties

Applications

Our Abpromise guarantee covers the use of ab55167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 64 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionGranulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
  • Tissue specificityIn myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
  • Involvement in diseaseDefects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
  • Sequence similaritiesBelongs to the granulin family.
  • Post-translational
    modifications
    Granulins are disulfide bridged.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrogranin antibody
    • CLN11 antibody
    • GEP antibody
    • GP88 antibody
    • Granulin A antibody
    • Granulin B antibody
    • Granulin C antibody
    • Granulin D antibody
    • Granulin E antibody
    • Granulin epithelin antibody
    • Granulin F antibody
    • Granulin G antibody
    • Granulin-7 antibody
    • Granulins antibody
    • GRN antibody
    • GRN_HUMAN antibody
    • PC cell derived growth factor antibody
    • PCDGF antibody
    • PEPI antibody
    • PGRN antibody
    • Proepithelin antibody
    • Progranulin antibody
    see all

Anti-Granulin antibody images



  • Predicted band size : 64 kDa
    Granulin antibody (ab55167) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55167 at 10 ug/ml staining Granulin in human Hela cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Granulin antibody (ab55167)

ab55167 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Methanol
Permeabilization Yes - methanol
Sample Human Cell (HELA cells)
Specification HELA cells
Gating Strategy Hela cells were gated for FSC and SSC parameters.
Preparation Cell harvesting/tissue preparation method: Rinse the cell layer with 0.25% (w/v) Trypsin- EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Sample buffer: Trypsin-EDTA solution
Username

Abcam user community

Verified customer

Submitted Feb 24 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Tissue sections (Brain tissues, fresh frozen)
Specification Brain tissues, fresh frozen
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"