Recombinant
RabMAb

Anti-Granulin antibody [EPR3781] (ab108608)

Overview

  • Product name
    Anti-Granulin antibody [EPR3781]
    See all Granulin primary antibodies
  • Description
    Rabbit monoclonal [EPR3781] to Granulin
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Granulin.

  • Positive control
    • WB: A431, 293T, MCF7 and HeLa cell lysates and human fetal lung and fetal kidney tissue lysates. IHC-P: Human kidney tissue. ICC/IF: Malme-3M cells. Flow Cyt: A431 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
WB 1/1000 - 1/10000. Detects a band of approximately 55, 75 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.

Target

  • Function
    Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
  • Tissue specificity
    In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
  • Involvement in disease
    Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
  • Sequence similarities
    Belongs to the granulin family.
  • Post-translational
    modifications
    Granulins are disulfide bridged.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrogranin antibody
    • CLN11 antibody
    • GEP antibody
    • GP88 antibody
    • Granulin A antibody
    • Granulin B antibody
    • Granulin C antibody
    • Granulin D antibody
    • Granulin E antibody
    • Granulin epithelin antibody
    • Granulin F antibody
    • Granulin G antibody
    • Granulin-7 antibody
    • Granulins antibody
    • GRN antibody
    • GRN_HUMAN antibody
    • PC cell derived growth factor antibody
    • PCDGF antibody
    • PEPI antibody
    • PGRN antibody
    • Proepithelin antibody
    • Progranulin antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108608 at 1/100.
  • All lanes : Anti-Granulin antibody [EPR3781] (ab108608) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Human fetal lung tissue lysate
    Lane 6 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 55,75 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab108608 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108608, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Flow cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Granulin (red) with ab108608 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

References

This product has been referenced in:
  • Qian L  et al. MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Mol Med Rep 14:3021-8 (2016). WB ; Human . Read more (PubMed: 27571908) »
  • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

For ab108608, the immunogen is within a.a. 40 - 60.

For ab140653, the imuunogen is within a.a. 570 - 590.

For ab53880, the immunogen is considered proprietary from the lab, but a BLAST search w...

Read More

Gracias por contactarnos de nuevo.

Sin duda en esta ocasión los resultados obtenidos son más satisfactorios, y corresponden con los obtenidos por nosotros en el laboratorio, en los que se aprecia una banda a 55KDa correspondient...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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