The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml. No special pretreatment is required.
IHC-Fr: Use at a concentration of 2 - 4 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells.
Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
References for Anti-Granulocytes antibody [0.N.315] (ab14497)
has not yet been referenced specifically in any publications.
Publishing research using ab14497? Please let us know so that we can cite the reference in this datasheet.
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