Anti-Granulocytes antibody [0.N.315] (ab14497)

Overview

  • Product nameAnti-Granulocytes antibody [0.N.315]
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [0.N.315] to Granulocytes
  • SpecificityRecognizes an unidentified antigen in the cytoplasm of mature granulocytes.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Nuclei from pokeweed mitogen-stimulated human peripheral blood lymphocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number0.N.315
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
ICC/IF
  • Application notesIF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml. No special pretreatment is required.
    IHC-Fr: Use at a concentration of 2 - 4 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

    References for Anti-Granulocytes antibody [0.N.315] (ab14497)

    ab14497 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"