Anti-Granulocytes antibody [HIS48] (ab33760)

Overview

  • Product nameAnti-Granulocytes antibody [HIS48]
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [HIS48] to Granulocytes
  • Specificityab3760 recognises granulocytes and erythroid cells.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    PVG rat spleen cell suspension.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tissue culture supernatant, 8% Foetal calf serum, 0.2M Tris HCl. pH 7.4
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberHIS48
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
Flow Cyt Use 10µl for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

References for Anti-Granulocytes antibody [HIS48] (ab33760)

ab33760 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The immunogen used for this antibody was not a specific peptide but rather a cell suspension from rat spleen. Unfortunately, we have not performed any detailed mapping on this clone and thus we currently do not h...

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Thank you for your inquiry. According to our records, the specificity of this clone is supported by the following papers:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783230/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793189/
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Thank you for your reply. I am sorry that I misunderstood your original email. The antibody ab33760 was generated from PVG rat spleen cell suspension and is specific to granulocytes and erythroid cells. Unfortunately I do not have any more information ...

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Thank you for contacting Abcam. The antibody ab33760, anti-Granulocytes antibody [HIS48] was generated from PVG rat spleen cell suspensions. The exact epitope sequence is proprietary and I do not have access to that information and therefore I cannot t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"